Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence
Figure 8
Exosome mediated delivery of miRNAs in bovine granulosa cells in vitro.
Purified exosomes from follicular fluid of follicles from growing (Exo BCB-) or fully grown (Exo BCB+) oocytes were co-cultured with bovine granulosa cell. After 24 hrs of incubation at 37°C in a humidified incubator, cells were collected and subjected to total RNA extraction. The expression levels of candidate miRNAs were investigated by real time PCR. In both cases the level of endogenous miRNAs were significantly increase compared to untreated controls. Bars with different superscript letters (a,b) are significantly different (P<0.05) from each other. The data is presented as means ± SD of three biological replicates.