Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence
Figure 6
Uptake of PKH67-labeled exosomes by bovine granulosa cells in vitro.
Exosomes, purified from follicular fluid, were labeled with PKH67 dye and added to primary culture of granulosa cells. Granulosa cells were co-cultured with labeled exosomes isolated from follicular fluid in exosome-free medium for 22 hours under optimum cell culture conditions (37°C & 5% CO2). Nuclei are stained blue (DAPI) while PKH67-labeled exosomes are stained green (Figures 7 A, B & C). Arrows indicate exosomes that were taken up by granulosa cells. Granulosa cells cultured in exosomes free medium containing PKH67-labeled sterile PBS served as a negative control (Figure 7 D). Scale bar: 10,000 nm.