Endoplasmic Reticulum Stress, Unfolded Protein Response and Altered T Cell Differentiation in Necrotizing Enterocolitis
Figure 1
Occurrence of ER stress and the UPR in a subset of A-NEC patients.
(A) Splicing of XBP1 mRNA was checked in all patients, and representative PCR products of XBP1u and XBP1s are shown using DNA electrophoresis. Dimethyl sulfoxide (DMSO)-treated HIEC cells were used as negative control for XBP1s, and TM-treated HIEC cells were used as positive control for XBP1s.
Mucosal mRNA expression levels of GRP78 (B) and CHOP (C) in the ileum of patients were quantified using qPCR and normalized to GAPDH mRNA levels. Asterisks indicate statistical significant differences between indicated groups.
(D) The Western blot result shows the representative protein expression of GRP78 and CHOP in A-NEC-XBP1u and A-NEC-XBP1s patients, and beta ACTIN was used as loading control.