The Suppressive Role and Aberrent Promoter Methylation of BTG3 in the Progression of Hepatocellular Carcinoma
Figure 5
BTG3 knockdown promotes proliferation, invasion and G1/S phase transition of HCC cells in vitro.
(A) BTG3 expression in BTG3 depleting 97H and 7701 cells by Western blot. (B) Effect of BTG3 knockdown on cell proliferation in vitro by MTT assay. (C) Effect of BTG3 knockdown on cell invasion in vitro by using Boyden chambers. (D) Morphological comparison of BTG3-depleting cells and control cells penetrating the artificial basement membrane was shown. (E) Effect of BTG3 kncokdown on cell cycle transition in vitro by flow cytometry. (F) Expression levels of Cyclin D1 and p27 in BTG3-overexpressing and depleting cells by Western blot. Tubulin was shown as a control.