An Exposure to the Oxidized DNA Enhances Both Instability of Genome and Survival in Cancer Cells
Figure 11
Activity of STAT3 is stimulated in MCF-7 cells exposed to either gDNA or gDNAOX at final concentrations of 50 ng/mL.
A FACS: Frequency plot for fluorescence intensities in cells stained with anti-STAT3 antibodies [1] and the average of the median signal intensities of FL1 (STAT3) in these cells [2].
B Fluorescent microscopy of cells stained with STAT3 antibodies (x20) [1]. - non-treated control cells and cells exposed to either gDNA or gDNAOX for 2 hours [2]. - cells pre-treated for 30 min by 0.15mM NAC, then exposed to either gDNA or gDNAOX for 2 hours.
С [1] - evidence for nuclear localization of STAT3 (x100), the nuclei were stained with DAPI [2]. - to evaluate the background, the cells were treated with normal rabbit IgG and FITC-conjugated secondary antibodies.