TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression
Figure 8
Prostate CAFs express a higher level of TIMP-1 receptor, CD63, and TIMP-1 promotes activation of ERK1/2 kinases in prostate CAFs.
A. Expression of CD63 protein in various human prostate cancer cell lines, human prostate fibroblasts (HPrF), and human prostate CAFs (PCAFs) was determined by western blotting using anti-CD63 antibody, which recognizes heterogeneous glycosylated CD63 protein. Actin was used as a control for protein loading (lower panel). B-C. Serum-starved prostate CAF cells (B) and 22RV1 prostate cancer cells (C) were supplied with serum free medium (SFM) or SFM containing 250ng/ml of TIMP-1 for 3 and 12 hours. The cells were lysed and proteins were analyzed by Western blotting using anti-phospho-ERK1/2 or anti-phospho-AKT antibodies (upper panels), or anti-ERK1/2 or anti-AKT antibodies (bottom panels).