Cell Infectivity in Relation to Bovine Leukemia Virus gp51 and p24 in Bovine Milk Exosomes
Figure 4
Reverse transcriptase activity in exosomes.
(A) Standard curve of RT activity constructed by serially diluted M-MLV RT (5, 2.5, 1.25, 0.625 and 0 units/5 µl). (B) Detection of RT activity in purified exosomes by immuno-magnetic separation. Significantly higher activity in purified exosomes/CD9/Dynabeads complexes than isotype IgG/Dynabeads complexes was observed by parametric non-repeated measures ANOVA and SNK test. *, P<0.01. Unb. Exo/9/B; unbound solution of exosomes/CD9/Dynabeads complexes. Unb. Iso/B; unbound solution from the mixture of exosomes and isotype IgG/Dynabeads complex. Exo/9/B; exosomes/CD9/Dynabeads complexes. Iso/B; magnetically separated pellet from the mixture of exosomes and isotype IgG/Dynabeads complex. Negative control; water.