A Novel M2e Based Flu Vaccine Formulation for Dogs
Figure 2
The M3M2 antigen mixed with the OmpC adjuvant induces a strong humoral response to the H3N8 M2e peptide.
Balb/C mice (10 per group) were immunized twice with either 100µg of the recombinant M3M2 antigen or with 23µg of the H3N8 M2e peptide. Total IgG titers (A) or IgG2a (B) directed to the H3N8 M2e peptide were evaluated by ELISA using serum collected 14 days after the second immunization. Using a similar immunization protocol, Balb/C mice were immunized with 20µg of M3M2 alone or mixed with one of several doses of the adjuvant OmpC (OC) (20, 40, 60 or 80µg) to increase the immune response to the M2e peptide. Total IgG (C) and IgG2a (D) were evaluated from serum collected 14 days after the second immunization. Finally, to evaluate if the vaccine formulations could trigger a memory response, ELISA was performed with serum collected at day 61 after the second immunization of the same animals as in C and D. Total IgG (E) and IgG2a (F) directed to the peptide H3N8 M2e were measured. Adjuvanted groups unified under one arc means that they are all significantly different from the non-adjuvanted group with the same level of confidence showed with the above capped line. * p< 0.05, ** p< 0.01, *** p< 0.001.