Characterization of the Cardiac Renin Angiotensin System in Oophorectomized and Estrogen-Replete mRen2.Lewis Rats
Figure 6
Plasma and cardiac Ang 1–7 and cardiac mas receptor.
Plasma Ang 1–7, cardiac Ang 1–7 and mas receptor expression were determined in left ventricles of sham-operated and oophorectomized female mRen2.Lewis rats treated with vehicle or estradiol for 4 weeks. (A) Plasma Ang 1–7 concentration, (B) Representative images showing Ang 1–7 staining in the left ventricles and the intensities quantified using ImageJ, (C) Mas receptor mRNA expression determined by real-time PCR, (D) Western blot for mas receptor in left ventricles. Values are mean ± SEM; * P<0.05 vs. sham, # P<0.05 vs. OVX.