MIP-2A Is a Novel Target of an Anilinoquinazoline Derivative for Inhibition of Tumour Cell Proliferation
Figure 2
Q15 directly binds to MIP-2A and inhibits the interaction between MIP-2A and MBP-1.
(A) Recombinant MIP-2A was expressed in E. coli and fractionated by gel-filtration chromatography. The fraction was then subjected to 15% SDS-PAGE followed by CBB staining (left). A representative biosensorgram of MIP-2A binding on SA sensor chips with immobilised biotinylated-Q15 is shown. The KD value was determined (right). (B) T7-MIP-2A and GST-MBP-1 were generated by in vitro translation and used in a pull-down assay with glutathione sepharose in the presence of 0–10 µM free Q15. Each fraction was separated by 15% SDS-PAGE and analysed by western blotting with an antibody against T7 tag or GST.