Effects of MicroRNAs on Fucosyltransferase 8 (FUT8) Expression in Hepatocarcinoma Cells
Figure 2
Effects of miRNA transfection HepG2 cells.
Cells were transfected with either the AllStar siRNA negative control, miR-122 mimic or miR-34a mimic in 12 well plates. After 24 and 48 hours total RNA and proteins were extracted. (A) mRNA levels were determined by qRT-PCR. Data were reported as relative fold expression compared to controls (indicated by the dotted line) and they were means ± SD of seven independent experiments. (B) Total proteins of HepG2 were recovered after 24 and 48 hours post-transfection of AllStar siRNA negative control, miR-122 or miR-34a mimics. After SDS-PAGE separation and blotting, the membranes were probed sequentially using antibodies against FUT8, ALDOA and β-Actin. Band intensities were measured by densitometry and the values obtained for FUT8 and ALDOA were normalized using β-Actin. Data are expressed as normalized levels relative to the cells transfected with the negative control (indicated by the dotted line). Data were obtained from three independent experiments. *p<0.05.