Effects of MicroRNAs on Fucosyltransferase 8 (FUT8) Expression in Hepatocarcinoma Cells
Figure 1
Effects of miR-122 and miR-34a on FUT8 and ALDOA 3′UTRs in a luciferase reporter assay.
For each experiment, HeLa cells were seeded in 96 plates (six replicates for each condition) and they were then co-transfected with either miR-122 or miR-34a mimics and with empty pMiR-Report, with pMiR-Report containing downstream FUT8 3′UTR (pMiR-Report/FUT8) or ALDOA 3′UTR (pMiR-Report/ALDOA). Luciferase activity was determined after 24 hours post-transfection. Control = AllStar siRNA negative control. Data were expressed as luminescence units (RLU) means ± SD of six replicates in three independent experiments. *p<0.05.