Identification and Structural-Functional Analysis of Cyclin-Dependent Kinases of the Cattle Tick Rhipicephalus (Boophilus) microplus
Figure 8
MTT viability assay of BME26 cells.
BME26 cells were incubated with different concentrations of roscovitine (a CDK inhibitor) or DMSO 0.1% (control) for 24 h (gray bar) and 48 h (grid bar) and then cell viability was determined by the MTT assay. After incubation the medium was removed and added MTT for 2 h. Reaction was read in spectrophotometer at 570 nm. Graph represents three independent experiments in triplicate. On all tested concentrations, with the exception of roscovitine 75 µM for 24 h, values of roscovitine groups were significantly different (One-way analysis of variance—ANOVA, p<0.05) as compared to the control groups.