Lactate Dehydrogenase A Expression Is Necessary to Sustain Rapid Angiogenesis of Pulmonary Microvascular Endothelium
Figure 3
LDH-A rescue promotes PMVEC growth.
Cells were grown in the absence of doxycycline for five days, and then on day six, doxycycline treatment was initiated. Doxycycline treatment rescued LDH-A protein (A), lactate production (B), glucose consumption (C), and PMVEC growth (D). One-way ANOVA was used to assess significance over the 12-day time course, two-way ANOVA was used to compare between groups, and Bonferroni post hoc test was performed as needed. Significant differences over time (^) and due to doxycycline treatment (*) are shown (P < 0.05). Data represent averages + SEM from 3-5 experiments per group, each performed in triplicate.