Lactate Dehydrogenase A Expression Is Necessary to Sustain Rapid Angiogenesis of Pulmonary Microvascular Endothelium
Figure 2
LDH-A is required for PMVECs to sustain aerobic glycolysis and rapid growth.
Western blot analysis demonstrates that LDH-A protein decreases in the absence of doxycycline over a 7-day time course (A) resulting in decreased lactate production (B), glucose consumption (C) and PMVEC growth (D). Panel A shows a representative western blot taken from three different experiments performed in parallel with the growth curve. Two-way ANOVA was used to compare between groups, and Bonferroni post hoc test was performed as needed. Significant differences over time (^) and between doxycycline treatments (*) are shown (P < 0.05). Data represent averages + SEM from 3-5 experiments per group, each performed in triplicate.