Development of a Cost-Effective Method for Capripoxvirus Genotyping Using Snapback Primer and dsDNA Intercalating Dye
Figure 1
Nucleotides sequence alignment of the RPO30 gene of CaPVs highlighting the snapback tail binding site.
The RPO30 gene sequences of 7 CaPVs representing GTPVs, LSDVs and SPPVs were aligned. A sequence of sixteen nucleotides complementary to the snapback tail in GTPV (100% match), as well as the corresponding positions in SPPV and LSDV are shown in the box. Note the targeted single nucleotide mismatches inside the box: T:A between GTPV and SPPV, and T:G between GTPV and LSDV. Conserved nucleotides are shown as dots.