Xanthine Oxidase Mediates Hypoxia-Inducible Factor-2α Degradation by Intermittent Hypoxia
Figure 4
Xanthine Oxidase (XO) activity mediates HIF-2α degradation by IH.
A–B Representative immunoblot of HIF-2α expression in cells exposed to IH in the presence of ALLO (A), and in cells transfected with XDH siRNA (B). C HIF-2α degradation in PC12cells treated with Xanthine (Xa)/XO (250 uM/0.01 U/ml) under normoxia, and the effect of MnTmPyP or ALLO co-treatment, respectively. Bottom panels of A, B and C show quantitative data of densitometric analysis presented as mean ± S.E.M. from 4 experiments, *p<0.05. n.s. not significant p>0.05.