Metagenomic Screening for Aromatic Compound-Responsive Transcriptional Regulators
Figure 3
Induction specificity of metagenomically retrieved transcriptional regulators.
Clones: A: pSAL7A/EcoRV, B: pSAL10D/EcoRI, C: pSALM2B/BsmI, D: pMECA2G/SmaI, E: pMECA5D/SacI, F: pMECA7B/EcoRV, G: pCHLO4C/SalI, H: pCHLO6C/NotI, and I: pCHLO8F/HincII. Induction compound: 1, none; 2, phenol; 3, catechol; 4, o-cresol; 5, m-cresol; 6, p-cresol; 7, 3-methylcatechol; 8, 4-methylcatechol; 9, 4-chlorocatechol; 10, benzoate; 11, salicylate; 12, 3-hydroxybenzoate; 13, 4-hydroxybenzoate; 14, 2-chlorobenzoate; 15, 3-chlorobenzoate; 16, 4-chlorobenzoate; 17, hydroquinone; 18, chlorohydroquinone; and 19, 4-chlororesorcinol. Fluorescence intensity was normalized to cell densities. Values represent the means of five measurements.