Filopodia and Membrane Blebs Drive Efficient Matrix Invasion of Macrophages Transformed by the Intracellular Parasite Theileria annulata
Figure 3
ERM proteins localize sequentially to leading edge, the neck zone and the trailing edge.
A) If microscopy analysis of ERM protein localization in cells migrating in matrigel using anti-ERM antibodies (red). Leading edge of cells was determined using cortactin localization (green). The location of the trailing edge was confirmed by visualizing host and parasite nuclei (arrows) using hoechst stain. Arrowheads indicate neck zones. B) Quantification of YFP-ezrin fluorescence intensity (leading to trailing edge). Top row shows still images of YFP-fluorescence of time lapsed-image acquisition for a period of 9 min. Bottom row are the corresponding gray-scale images. Middle row shows intensity histograms of YFP-fluorescence along the white line (top row left). Arrows indicate tail, arrowhead leading edge.