Low-Density Lipoprotein Receptor Related protein-1 (LRP1)-Dependent Cell Signaling Promotes Neurotrophic Activity in Embryonic Sensory Neurons
Figure 4
RBD-induced neurite sprouting is blocked in primary sensory neurons pre-treated with a Src-family kinase inhibitor, PP2.
A, Phase-contrast images of cultured sensory neurons after 2 weeks of treatment. Note the reduction of sprouting in RBD (100 nM) treated cultures, but not NGF (50 ng/ml) treated cultures with treatment of PP2 (1 µM) for 30 min prior to the addition of factors. Scale bar, 50 µm. B, Levels of GAP-43 mRNA in primary cultures treated with RBD (100 nM) or NGF (50 ng/ml) for 2 weeks, with or without PP2 treatment. *p<0.05 (n=3-4 independent experiments).