Effects of Specific Multi-Nutrient Enriched Diets on Cerebral Metabolism, Cognition and Neuropathology in AβPPswe-PS1dE9 Mice
Figure 6
Neurogenesis in 12-month-old AβPP-PS1 and wild-type mice on control and specific multi-nutrient diets.
From 2 months of age, mice were fed either a control (CO), a DHA, EPA and UMP (DEU) or a Fortasyn® Connect (FC) diet. The amount of immature neurons in the subgranular zone of the hippocampus were visualized immunohistochemically with a polyclonal goat anti-doublecortin antibody (1:3000) as a measure for neurogenesis. Values represent the mean and SEM and are relative (%) compared to wild-type mice on CO diet. In wild-type animals, the relative amount of doublecortin-positive (Dcx+) immature neurons was similar for all diet groups. However, in AβPP-PS1 mice the relative amount of Dcx+ immature neurons was affected by dietary intake (p = 0.017). Post hoc analysis revealed that AβPP-PS1 mice fed the CO diet displayed a significantly decreased relative amount of Dcx+ immature neurons compared to wild-type mice on the CO diet (*p = 0.004). The FC diet significantly increased the relative amount of Dcx+ immature neurons in AβPP-PS1 mice as compared to AβPP-PS1 mice on CO diet (*p = 0.015). Furthermore, AβPP-PS1 mice on the FC diet had slightly higher relative amount of Dcx+ immature neurons than wild-type animals on the FC diet (#p = 0.053), although this did not reach statistical significance.