Biodistribution and Subcellular Localization of an Unnatural Boron-Containing Amino Acid (Cis-ABCPC) by Imaging Secondary Ion Mass Spectrometry for Neutron Capture Therapy of Melanomas and Gliomas
Figure 4
SIMS analyses of brain from F98 glioma bearing rats.
Rats received cis-ABCPC by i.p. at a dose of 250 mg/kg b.w. and were euthanized 2.5 hr. later. The morphology of F98 glioma is shown in H & E stained cryosections (A-B). The main tumor mass (TM), normal brain tissue (BT), clusters of tumor satellite cells (TS) and individual infiltrating tumor cells (TC) are discernible in normal brain tissue (A). The optical image (B) shows a vessel (V) which is surrounded by TC and BT. SIMS analyses were made in adjacent cryosections. SIMS imaging analyses of boron from two different tissue regions are shown in C-D and E-F, respectively. In C-D, a subtle gradient of 39K+ in the SIMS image identified the boundary of the TM from the BT which contains a TS and TC’s in panel C. The respective boron microdistribution from cis-ABCPC is shown in the 11B+ boron SIMS image in panel D: the higher concentrations of boron in TM, TS, and TC’s are revealed in comparison to the BT. In a second example of the SIMS imaging of another tissue region (E-F), a gradient of 24Mg+ is used for the identification of TS and infiltrating TC’s in the normal brain tissue which also contains a vessel (V) surrounded by TC’s (E). The respective 11B+ SIMS image from this region, shown in F, revealed higher concentrations of boron in TS as well as the infiltrating TC’s in the normal brain tissue surrounding the vessel (V). The image integration times on the CCD camera for SIMS images of 39K+ and 11B+ in C and D were 0.2 sec and 2 min, respectively. The image integration times on the CCD camera for SIMS images of 24Mg+ and 11B+ in E and F were 1 min and 2 min, respectively.