Dynamics of the Major Histocompatibility Complex Class I Processing and Presentation Pathway in the Course of Malaria Parasite Development in Human Hepatocytes: Implications for Vaccine Development
Figure 6
Kinetic analysis of AS-mediated effects on MHC class I expression in infected hepatocytes.
HC-04 cultures infected with P. berghei ANKA GFP were exposed to activated supernatant (AS, 10% v/v) at the indicated time points post-infection and surface MHC class I staining was performed at 48 hrs post-infection. (A) Dot plots (GFP-) and contour plots (GFP+) represent MHC class staining with an APC-labeled MHC class I specific antibody or relevant isotype control antibody on infected and uninfected hepatocytes, respectively. One representative of 3 experiments is shown. The numbers indicate MFI values of MHC class I specific staining for respective conditions and populations. The dotted line boxes mark the observed area of events distribution in untreated cultures. (B) Summary of 3 experiments performed as described in A. The means ± SD of MFI values for surface MHC class I staining are shown. All p values are ≤0.01 and indicate differences between control and treated uninfected(*), GFP negative (**) and GFP positive (***) cells.