A Deficiency of Herp, an Endoplasmic Reticulum Stress Protein, Suppresses Atherosclerosis in ApoE Knockout Mice by Attenuating Inflammatory Responses
Figure 1
Herp expression in the artery.
A: The amount for Herp mRNA was determined by a real-time PCR in the aortas of apoE−/− mice (open columns) and Herp−/−; apoE−/−mice (closed columns). Each mRNA expression level was normalized to that of GAPDH. The mRNA levels are shown as relative ratios to the mRNA level of eight-week-old apoE−/− mice. Each value represents the mean ± SEM of five mice. N.D. indicates non-detectable. ** p<0.01 vs. apoE−/− mice at eight weeks of age. B: Immunostaining of Herp in the normal (upper panels) and atherosclerotic (lower panels) aortas of apoE−/− and Herp−/−; apoE−/− mice at 72 weeks of age. Blue; hematoxylin, Red; Herp (diaminobenzidine; DAB). The bar shows 50 μm.