Global Substrate Profiling of Proteases in Human Neutrophil Extracellular Traps Reveals Consensus Motif Predominantly Contributed by Elastase
Figure 2
Determination of the proteolytic signatures in NETs.
A-C. IceLogos representing the P4 to P4′ sites for NETs isolated from three donor samples. Amino acids that are most frequently observed (above axis) and least frequently observed (below axis) are illustrated. The number of cleavage sites used to make each iceLogo are listed in the bottom right-hand corner. Residues that are highlighted in black text are significantly (p = 0.05) enriched relative to the frequency that these same amino acids are found in the peptide library (5.2 +/- 0.5%). The amino acid ‘n’ corresponds to norleucine. D. Determination of the number of cleavage sites that are common and unique to each donor sample. E. Positional frequency of all donor-derived cleavage sites within the tetradecapeptides (n=85).