The Role of Mitochondria-Derived Reactive Oxygen Species in Hyperthermia-Induced Platelet Apoptosis
Figure 4
Effect of Mito-TEMPO on Bax mitochondrial translocation and cytochrome C release in hyperthermia-treated platelets.
(A–D) Washed platelets were incubated at different temperatures for 3 h (A and B), or pre-incubated with Mito-TEMPO and solvent control at 37°C for 15 min and then incubated at 42°C for 3 h (C and D). Treated platelets were lysed, and cytosol and mitochondrial fractions were isolated and analyzed by Western blot with anti-Bax (A and C), and anti-cytochrome C (B and D). Cytochrome C oxidase subunit 1 (COX1) and tubulin were used as internal controls. Representative data of three independent experiments are presented. Cytochrome C is labeled as Cyto C.