Anti-Tumor Effect of Adipose Tissue Derived-Mesenchymal Stem Cells Expressing Interferon-β and Treatment with Cisplatin in a Xenograft Mouse Model for Canine Melanoma
Figure 6
Cell cycle arrest of LMeC indirect co-cultured with cAT-MSC-IFN-β.
The cell cycle phase distribution of LMeC was analyzed after harvest by flow cytometery. (A, B) cAT-MSC:LMeC 1∶1 and (C, D) 4∶1. The LMeC cells co-cultured with cAT-MSC-IFN-β, showed increase in the G0/G1 phase of the cell cycle compared to the controls (p<0.05 and p<0.01 at a cAT-MSC-IFN-β/LMeC ratio of 1∶1 and 4∶1 respectively). G1 arrest occurred concurrently with a reduction in the percentage of S phase cells (p<0.01 and p<0.001 at a cAT-MSC-IFN-β/LMeC ratio of 1∶1 and 4∶1 respectively). The P-value was obtained using one-way ANOVA with post-hoc Bonferroni's multiple comparison by comparing treated cAT-MSCs (cAT-MSC-Mock or cAT-MSC-IFN-β) with control (LMeC alone) for each phase of the cell cycle. Data are representative of three independent experiments with similar results. * p<0.05, ** p<0.01, *** p<0.001.