Identification and Characterization of a Novel Plasmodium falciparum Adhesin Involved in Erythrocyte Invasion
Figure 7
Invasion inhibitory activity of PfMA antibodies.
PfMA antibodies were evaluated for their invasion inhibition activity against the P. falciparum 3D7 clone. Purified rabbit IgG at a concentration of 20 mg/ml was tested with different enzyme treated erythrocytes. The antibodies exhibited 35% inhibition of untreated erythrocytes (U), 54.6% inhibition of neuraminidase treated erythrocytes (N), 44.95% inhibition of trypsin (T) treated erythrocytes and 42.3% inhibition of chymotrypsin (C) treated erythrocytes. The control PfAMA-1 antibodies at 5mg/ml exhibited 71% inhibition. Three independent experiments were done in duplicate. The error bars represent the standard error of the mean between the assays. Statistical significance between invasion inhibition of untreated and enzyme-treated erythrocytes was calculated by the Student’s t-test with a p value < 0.05 indicating statistical significance (*p < 0.05; ** p < 0.01).