PRMT5 Is Upregulated in Malignant and Metastatic Melanoma and Regulates Expression of MITF and p27Kip1
Figure 2
PRMT5 is present and displays heterogeneous localization between human cell lines and orthotopic tumors.
A, Detection of PRMT5 in cell lysates from immortalized human epidermal melanocytes (HEM) or a panel of human metastatic melanoma cell lines. β-actin was used as loading control. B, HEM and melanoma cell lines underwent subcellular fractionation and immunoblot analysis for PRMT5, Mep50, or markers for nuclear (lamin B) and cytoplasmic (GAPDH) fractions. C, 1×106 cells from the A375 and Hs294T human melanoma cell lines were implanted into the flank of immunocompromised mice and grown for 21 days. Tumors were removed, fixed in formalin, and analyzed via IHC for PRMT5. Data represent total percentage of cells in each tumor positive for PRMT5 in nucleus, cytoplasm, or both. n≥2 tumors were analyzed.