Tumor and Endothelial Cell-Derived Microvesicles Carry Distinct CEACAMs and Influence T-Cell Behavior
Figure 5
CHO- and CHO-CEACAM1 derived MVs induce the CEACAM1-L tyrosine phosphorylation in confluent HT29 cells.
CEACAM1 immunoprecipitates of confluent HT29 cells treated for 15 min with CHO- and CHO-CEACAM1 derived MVs were probed for tyrosine phosphorylation (upper panel) and CEACAM1 (lower panel) as control for equal loading. Untreated cells were used as negative control. Pervanadate was used to induce CEACAM1-L tyrosine phosphorylation. The data show one representative result of three independent repeats of the experiment.