Hypertonic Saline Solution Drives Neutrophil from Bystander Organ to Infectious Site in Polymicrobial Sepsis: A Cecal Ligation and Puncture Model
Figure 1
Neutrophil Infiltration in lung.
CLP was induced in mice (n = 8 in each group), there were three groups: 1- only CLP (white); 2- 30 min later treated with hypertonic saline solution 7.5% (CLP+H) (black) or 3- treated with normal saline solution 0.9% (CLP+S) (gray). Forth group (C) was used to indicate basal levels. Samples from lung were harvested after 6h and 24h. CLP induced a significant increase in MPO, most notably in lung. Hypertonic solution treatment exhibited a significant reduction in MPO of lung (a). The adhesion molecule ICAM-1 increased after CLP procedure, and hypertonic saline solution presented a significant reduction in the amount of this molecule in lung (b). CXCL-1 presented an important increase after CLP, and hypertonic solution treatment reduced the amount of CXCL-1 in lung (c). Neither groups nor period show any difference in expression of GRK2 (d). CXCR2 expression was increased after CLP procedure and treatment with normal saline, and hypertonic saline solution reduces this expression (e). Data shown represent mean ± SEM. * p<0.05 indicates a significant difference with CLP control; # p<0.05 indicates a significant difference with CLP-S.