A Critical Role for Proinflammatory Behavior of Smooth Muscle Cells in Hemodynamic Initiation of Intracranial Aneurysm
Figure 8
SMCs express increased inflammatory markers at the BT 5 days after ligation.
BTs and BAs were harvested from 11 rabbits 5 days after ligation and from 7 rabbits 5 days after sham surgery, then stained for the inflammatory markers, MCP-1 and NF-κB. (A) Immunofluorescence of MCP-1 in an area of aneurysmal damage at the BT (left) compared to BA (right). (B) Immunofluorescence of NF-κB in an area of aneurysmal damage at the BT (left) compared to BA (right). (C,D) Average intensity of immunostaining shows that MCP-1 and NF-κB levels, respectively, are significantly higher in SMCs in the BT of animals after flow increase compared to the BA of the same animals and compared to levels in sham operated animals (* p<0.05). Scale bar = 50 µm. IU = intensity unit.