Impaired Cardiac SIRT1 Activity by Carbonyl Stress Contributes to Aging-Related Ischemic Intolerance
Figure 3
Alda-1 treatment protected cardiomyocytes against H/R injury.
Cardiomyocytes were pretreated for 1 h with vehicle, Alda-1(20 µmol/L) or Alda-1 plus Ex-527 (10 µmol/L), and then with or without 1 hr of hypoxia and 1 hr reoxygenation (H/R). Quantification showing (A) endogenous 4-HNE adduct protein, (B) cardiomyocytes caspase-3 activity, (C) cardiomyocyte death. (n=8 per group. *P<0.05 vs. vehicle control; #P <0.05 vs. vehicle H/R, † P <0.05 vs. Alda-1 H/R).