An Improved Quantitative Approach for the Assessment of Mitochondrial Fragmentation in Chemoresistant Ovarian Cancer Cells
Figure 5
CDDP- and piperlongumine-induced mitochondrial fission and apoptosis is Drp1-dependent in chemosensitive OVCA cells.
(A) CDDP (10 µM) and piperlongumine (10 µM) down-regulated of phospho-Drp1 (Ser637) content in OV2008 cells in vitro. (B) (i) Effect of mDivi-1 (0–10 µM) on CDDP- and piperlongumine-induced mitochondrial fission and (ii) apoptosis as assessed by Annexin V assay (**, p<0.01; ***, p<0.001 versus respective DMSO control treated with identical mDivi-1 concentration, #, p<0.05 versus respective PL or CDDP treatment in the absence of mDivi-1).