Quantitation of Gene Expression in Formaldehyde-Fixed and Fluorescence-Activated Sorted Cells
Figure 2
Comparison of mRNA quantitation by RT-qPCR between unfixed and fixed cells.
18S (A), GAPDH (B), TNFα (C) and MxA (D) mRNA quantitation by RT-qPCR were compared between unfixed and fixed samples of two cell lines, K562 and CEMx174, and human PBMCs. Individual points represent the difference of Ct values between unfixed and fixed samples for each gene obtained from one experiment (ΔCt = Fixed Ct – Unfixed Ct). Three biological replicates are shown for each sample. p values were calculated by a one-sample, two-tailed t test comparing the average ΔCt to a theoretical mean set to zero. Statistically significant p values are shown.