Toll-Like Receptor 4–Mediated Nuclear Factor Kappa B Activation Is Essential for Sensing Exogenous Oxidants to Propagate and Maintain Oxidative/Nitrosative Cellular Stress
Figure 3
Effect of anti-oxidants on secreted embryonic alkaline phosphatase (SEAP) release in pro-oxidant-stimulated HEK-Blue mTLR4 cells.
Cells were pre-treated with Ebselen (50 µM) or PBN (100 µM) followed by stimulation with MPLA (57 nM), PPC (1 µM), PDO (50 µM) or PN (1 mM) for 16 h in the continued presence of the anti-oxidants. SEAP release in the supernatant was determined using Quanti-Blue, and absorbance was read at 650 nm. The color-coded column in each panel (A to D) represents treatment with MPLA, PPC, PDO or PN alone, respectively, in the absence of the anti-oxidants. [The data represent n = mean ± SEM in 6 independent experiments; *p≤0.01 compared to MPLA treatment alone; **p≤0.001 compared with MPLA treatment alone; #p≤0.001 vs treatment with PPC alone; +p≤0.001 vs treatment with PDO alone; $p≤0.001 vs treatments with PN alone].