Functional Characterization of a Juvenile Hormone Esterase Related Gene in the Moth Sesamia nonagrioides through RNA Interference
Figure 4
Phenotypic results after hemolymph administration of dsJHERs.
A. Left: Normal Pupa, Right: Normal 6th instar d9 larva (Prepupa). B. Targeting the SnJHER 472 bp (BI.) or the SnJHER 1276 bp (BII.) or the SnJHER 1725 bp (BIII.) part after hemolymph administration of dsJHERs in 6th instar d9 larvae. BI. Resulted larval-pupal intermediates after hemolymph administration of dsJHER472 in 6th instar d9 larvae, BII. Resulted larval-pupal intermediates after hemolymph administration of dsJHER1276 in 6th instar d9 larvae, BIII. Resulted larval-pupal intermediates after hemolymph administration of dsJHER1725 in 6th instar d9 larvae. The arrows indicate similar abnormalities observed in intermediates of all targeted regions. In each case, -I-, -II- or -III- the abnormalities were presented in an average of >90% of the injected animals, 3 days post injection, (N = 100). C. Developmental abnormalities of dsJHER1725 5th instar d3 injected larvae. i. Normal larva, ii. Abnormal larva with fused (double) melanized epidermis of the previous instar. iii. Lateral view of abnormal larvae with fused epidermis. iv. The same larva of previous case -iii.-, by removing the melanized epidermis. The arrow indicates the fusion point between the previous and the new epidermal tissues. The newest epidermis is of yellowish colour. Scale bar: 1 cm