Characterization of GABAergic Neurons in the Mouse Lateral Septum: A Double Fluorescence In Situ Hybridization and Immunohistochemical Study Using Tyramide Signal Amplification
Figure 9
Double fluorescence in situ hybridization and immunohistochemistry labeling of neurons expressing mRNAs for GAD65/GAD67 (GAD) and NeuN-immunoreactivity in the Cg (A–F) and MPOA (G–L).
Low magnification images of the white boxed regions (A–C, G–I) show the GAD- and NeuN-expressing neurons were counted for colocalization analysis. High magnification images show the colocalization of GAD- and NeuN-expressing neurons in the Cg (D–F) and MPOA (J–L). Two typical examples of double-labeled neurons in the Cg and MPOA are indicated in arrows. Note that GAD and NeuN are highly coexpressed in a single cell. Scale bars = 150 µm in A-C, G-I; 50 µm in D–F, J-L.