Restoration of the Normal Splicing Pattern of the PLP1 Gene by Means of an Antisense Oligonucleotide Directed against an Exonic Mutation
Figure 2
RT-PCR from Oli-neu cells transfected with the wild-type and mutated minigenes.
Lane U: untransfected cells; lane WT: cells transfected with the wild-type minigene construct; lane SM: cells transfected with the minigene construct harbouring the c.436C>G mutation; lane DM: cells transfected with the minigene construct harbouring the double mutation [c.436C>T plus c.437T>A]; lane C: negative (no template) control; lane M: φX174 DNA HaeIII-restricted molecular weight marker. The minigene-specific primer 31GF/LACT2R-mediated RT-PCR products are 711 bp (PLP product) and 606 bp (DM20 product) in length, respectively. The asterisk denotes samples transfected with the pcDNA3.1/V5-His-TOPO/LacZ-PLP1-LacZ vector.