Efficient TALEN Construction for Bombyx mori Gene Targeting
Figure 2
(A) Positions of TALEN targets in the BmBLOS2 gene. Open rectangles and numbers above represent exons and their length in bp. The filled box is the 5′- UTR. The sequence above the line indicates part of exon 2 with BLTC target sites and their names. The sequence below the line indicates the third exon with BLT-2 and BLTS targets. Bgl II restriction site is underlined. The c above the Bgl II site indicates polymorphism in w1-pnd strain. (B) Positions of TALEN targets in the Bm-re gene. Open rectangles and numbers above represent exons and their lengths in bp. Filled boxes are 5′-and 3′ UTRs. The sequence below the line indicates the ninth exon (capital letters). Bgl II restriction site is underlined.