Low Seroprevalent Species D Adenovirus Vectors as Influenza Vaccines
Figure 2
Vaccine efficacy of species C and D Ad vectors by high dose systemic immunization.
Groups of 5 BALB/c mice were administered 1010 viral particles of the indicated vectors intramuscularly. All viral vectors contained a transgene cassette that expressed a soluble hemagglutinin (HA) derived from the influenza strain A/PR/8/34. Three weeks after immunization the mice were anesthetized i.p. with ketamine (140 mg/kg)/xylazine (5.55 mg/kg). The mice were challenged intranasally with 100MLD50 of influenza A/PR/8/34. The mice were weighed for baseline measurements. Included in the sacrifice criteria was 25% body weight loss.