Identification of New Dystroglycan Complexes in Skeletal Muscle
Figure 2
Nonmuscle cells within adult muscle do not significantly contribute to overall β-dystroglycan expression.
A. Representative Western blot of β -dystroglycan (βDG) expression in total skeletal muscle lysates from wild type (WT) mice and from P3Pro-Cre;Dag1 loxP/loxP mice that specifically lack dystroglycan expression in muscle cells (Dag1loxp). GAPDH was used as a loading control. Experiments were performed in triplicates. B. Representative Western blot of β-dystroglycan immunoprecipitations from wild type (WT) or dystroglycan deficient (Dag1loxp) skeletal muscle lysates probed for β-dystroglycan. Experiments were performed in triplicates. C. Representative Western blot of β-dystroglycan immunoprecipitations from wild type muscle for the presence of Dp71. No proteins were detected in non-specific antibody control immunoprecipitations (Ctr IP). Experiments were performed in duplicates.