Development of Agrobacterium-Mediated Virus-Induced Gene Silencing and Performance Evaluation of Four Marker Genes in Gossypium barbadense
Figure 1
Optimal factors for Agrobacterium-mediated GaPDS VIGS in G. barbadense.
A, The photobleaching phenotype of cotton leaves triggered by GaPDS VIGS. The pYL156 vector was used as a vector control; WT, wild type. B–E, The percentage of plants showing photobleaching was affected by light intensity, photoperiod, seedling age, and OD value of Agrobacterium cultures. Means ± standard deviation labeled with different letters are significantly different at the 0.05 level.