Aflatoxin B1 Negatively Regulates Wnt/β-Catenin Signaling Pathway through Activating miR-33a
Figure 3
miR-33a decreases the post-transcriptional activity of β-catenin and related genes in the β-catenin signaling pathway.
After transfection of pSilence 4.1-miR-33a or pSilence 4.1 vector (NC) into Chang liver and HepG2 cells for 48 h, cells were collected for the extraction of total RNA for real-time PCR. mRNA levels of all genes were normalized to β2-MG (control). (A) Relative expression level of miR-33a was confirmed by way of real-time PCR. (B) Overexpression of miR-33a down-regulates mRNA levels of β-catenin in Chang liver and HepG2 cells. mRNA expression patterns of the related genes in β-catenin signaling pathway were analyzed in Chang liver (C) and HepG2 (D) cells with miR-33a overexpression. Data is presented as mean ± SD. *P < 0.05, **P < 0.01, ***P <0.001.