Aflatoxin B1 Negatively Regulates Wnt/β-Catenin Signaling Pathway through Activating miR-33a
Figure 2
miR-33a negatively regulates β-catenin levels in cells.
(A) β-catenin protein levels in Chang liver, HeLa and AGS cells transfected with different miRNA expression vectors for 48 h. (B) Relative levels of the β-catenin protein after transfection for 48 h in Chang liver, HeLa and AGS cells. The β-catenin protein levels were normalized with β-actin in (A). (C) miR-33a down-regulates β-catenin protein levels in HepG2 cells after transfection for 48 h. (D) Relative levels of the β-catenin protein after transfection for 48 h in HepG2 cells. The β-catenin protein levels were normalized with β-actin in (C). Data is presented as mean ± SD. *P < 0.05, **P < 0.01, ***P <0.001.