Quercetin Inhibits Left Ventricular Hypertrophy in Spontaneously Hypertensive Rats and Inhibits Angiotensin II-Induced H9C2 Cells Hypertrophy by Enhancing PPAR-γ Expression and Suppressing AP-1 Activity
Figure 4
Histopathological analysis reveals that quercetin suppresses myocardial hypertrophy in spontaneously hypertensive rats.
Tissue sections were stained with hematoxylin and eosin (H&E; a1–a4; ×400) and Masson's trichrome (b1–b4, ×400). (c) Mean cardiomyocyte diameter was determined by measuring 100 cells from each section. (d) The ratio of the area of myocardial fibrosis (excluding perivascular collagen) to the total myocardial area (percentage) was quantified from images of Masson's trichrome-stained tissue. The scale bar denotes 100 µm. Both mean cardiomyocyte diameter and collagen volume were quantified using Image-Pro Plus software. At least 8–10 areas were evaluated in each section and each value denotes the mean ± sem (n = 8 in each group). CVF, collagen volume fraction. *P<0.05, vs. the Wistar–Kyoto rats (WKY) group; **P<0.05, vs. the spontaneously hypertensive rats (SHR) group; #P<0.05, vs. the SHR+QH group.