Chimeric Newcastle Disease Virus Protects Chickens against Avian Influenza in the Presence of Maternally Derived NDV Immunity
Figure 6
Virus shedding after vaccination.
Swab samples taken on the indicated days from oropharynx of MDA- chickens (A) immunized with chNDVFHN PMV8H5 at three weeks of age and swabs combined from oropharynx and cloaca of MDA+ chickens (B) immunized on day 1 (group a) or day 7 (group b) after hatch were analyzed for the presence of NDV NP gene-specific RNA by RT-qPCR. Values were transformed to genome equivalents (GEQ) using calibration curves of defined RNA standards that were included with each RT-qPCR run. The number of positive swabs by RT-qPCR is given below the box plots. Significant differences (P < 0.0125, Bonferroni correction) between vaccinated groups and controls are indicated (*).