Expression of Galectin-7 Is Induced in Breast Cancer Cells by Mutant p53
Figure 7
Galectin-7 expression is dependent of NF-κB signalling pathway.
mRNA levels following treatment with increasing doses of TNFα (A) or at different time post-treatment with TNFα (B). (C) In silico analysis showing effect of parthenolide on TNFα-induced galectin-7 expression in epidermal keratinocytes. Data were retrieved from a dataset (GDS : 1289) in the Gene Expression Omnibus (GEO) repository. (D) Effect of parthenolide on galectin-7 expression in HaCaT cells. mRNA levels of galectin-7 was measured by semi-quantitative RT-PCR analysis 48h after treatment. No such effect was observed on C/EBPβ expression. GAPDH was used as loading control. (E) Dose-dependent inhibition of galectin-7 mRNA levels in MDA-MB-468 cells treated with CAPE. GAPDH was used as loading control. (F) Dose-dependent inhibition of galectin-7 mRNA levels in MDA-MB-468 cells treated with quercetin. GAPDH was used as loading control.