Comparing Adjuvanted H28 and Modified Vaccinia Virus Ankara Expressing H28 in a Mouse and a Non-Human Primate Tuberculosis Model
Figure 4
Correlation between T cell subsets induced pre and post infection and CFU.
A, The splenic CD4 T cell responses obtained using ICS and flow cytometry one week after immunizations shown in figure 2 and S2 (Ag85B- and TB10.4-stimulation) were correlated to the corresponding mean log10 CFU value obtained in the lungs six weeks after infection. Points represent mean percentage +/− SEM (vertical) of CD4 T cells producing different combinations of IFN-γ, TNFα, IL-2 in response to stimulation with indicated antigens from 4 spleens per group plotted on the y-axis and mean +/− SEM (horizontal) log10 CFU values of individual mice on the x-axis. Each point represents one group as indicated in the graph. B, the pulmonary CD4 T cell responses obtained using intracellular cytokine staining (ICS) and flow cytometry six weeks after infection shown in figure 3 and S3 (Ag85B- and TB10.4-stimulation) were correlated to the corresponding mean log10 CFU values within each group. Points represent individual mean +/− SEM values as described in A. *p<0.05, **p<0.01, using Pearson’s product-moment correlation coefficient (r) and correlation test. T cell subsets that showed negative correlation with CFU are marked with a black star, whereas those that showed positive correlation are marked with a white star.