A Human Antibody to the CD4 Binding Site of gp120 Capable of Highly Potent but Sporadic Cross Clade Neutralization of Primary HIV-1
Figure 5
1F7 binding to sensitive and resistant Envs.
Binding of 1F7 and other mAbs to detergent-solubilized Env from different isolates of pseudotyped HIV-1: (A) JR-FL, (B) JR-CSF, (C) MN, and (D) 93MW959. Env A (JR-FL) is 1F7-sensitive whereas Envs B-D are 1F7-resistant. Curves were analyzed with GraphPad Prism 5.04 using a four-parameter fit with shared values for minimum binding. Data are representative of at least 3 titrations of each MAb against the respective detergent-solubilized Env.